Analysis Note

Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Application

Lectin from Wisteria floribunda has been used:to study the perineuronal nets and parvalbumin nets in mouse brain by immunofluorescenceto evaluate the amount of N-acetylgalactosamine (GalNAc) transferred to Gaussialuciferase (GLuc) substrates by microplate assayto study its binding effects on perineuronal nets

Biochem/physiol Actions

Lectin binds with high affinity to the glycans of polysaccharides, glycolipids, and glycoproteins. It is involved in plant defense.

WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.

General description

Lectin is a carbohydrate-binding protein found in plant roots, rhizomes, leaves, seeds, and stems. Lectin is located in the cytoplasm as well as in the nucleus.

Packaging

Package size based on protein content

1, 5 mg in glass bottle